Thirteen new microsatellite loci in Rhagoletis cerasi (Diptera: Tephritidae), a model host species for Wolbachia symbiosis in field populations


Arthofer W., Krumböck S., Schuler H., Rasool B., Riegler M., Köppler K. and Stauffer C.


The European cherry fruit fly Rhagoletis cerasi, an important pest in cherry orchards, exhibits Wolbachia - induced crossing incompatibilities between two geographic complexes in Europe. Here we present 13 new microsatellite markers in R. cerasi. All markers are polymorphic with 4 to 12 alleles per locus and observed heterozygosity values between 0.25 and 0.95. Two markers show significant deviations from Hardy-Weinberg proportions after Bonferroni correction. While the influence of Wolbachia on the host's mitochondrial genome is recognised, the markers presented here will provide a useful tool for the analysis of population structure and dynamics of this multiply infected field model species for the first time.



Molecular Ecology Resources 9, 1460-1466.




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